摘要
目的 :RP -HPLC法测定人血浆中苯巴比妥、苯妥英和卡马西平浓度。方法 :采用美国Waters公司6 80型高效液相色谱仪 ,4 86紫外可变波长检测器 ,UltrasphereODS (15 0× 4 6mm ,5 μm)色谱柱 ,柱温为室温 ,用甲醇 :0 0 1mol·L-1磷酸二氢钾 =5 0∶5 0 (pH5 6 3)为流动相 ,酮洛芬为内标 ,在 2 4 5nm处检测。结果 :标准曲线线性范围分别为 :苯巴比妥 :12 5～ 2 0 0 0 μmol·L-1;苯妥英 :6 2 5～ 10 0 0 μmol·L-1;卡马西平 :5 0～ 80 0 μmol·L-1;萃取回收率分别为苯巴比妥 92 6 %～ 95 1% ,苯妥英 92 9%～ 93 3% ,卡马西平 91 0 %～ 92 9% ;方法回收率为苯巴比妥 93 0 %～ 10 6 9% ,苯妥英 92 9%～ 95 5 % ,卡马西平 92 9%～ 10 4 2 % ;日内测定RSD :苯巴比妥2 9%～ 9 2 % ,苯妥英 3 3%～ 9 6 % ,卡马西平 3 4 %～ 9 9% ,日间测定RSD苯巴比妥 2 2 %～ 9 5 % ,苯妥英1 8%～ 9 8% ,卡马西平 2 6 %～ 9 4 %。结论 :本办法快速、灵敏、准确 ,可用于临床同时监测三种药物浓度。
Objective:To develop a method for the determination of the concentrations of Phenobarbital,Phenytoine and Carbamazepin in human plasma by RP-HPLC.Methods:The plasma sample was extracted with 2ml ethyl acetate(CH 3COOCH 2CH 3),then separation was achieved on the Ultrasphere ODS(150×4.6mm,5μm)column.The mobile phase was consisted of CH 3OH-0.01mol·L -1 NaH 2PO 4=50∶50(pH5.63)and the effluent was measured at UV 245nm.Ketoprofen was used as the internal standard.Results:The retention times for Phenobarbital,Phenytoine,Carbamazepin and Ketoprofen were 3.64min?8.11min and 5.63min respectively.The calibration curves of Phenobarbital,Phenytoine and Carbamazepin were linear over the range of 12.5～200.0μ mol·L -1 ,6.25～100.0μ mol·L -1 and 5.0～80.0μ mol·L -1 respectively.The extraction recovery of Phenobarbital,Phenytoine and Carbamazepin were 92.6%～95.1%,92.9%～93.3%and 91.0%～92.9%.The method recovery were 93.0%～106.9%,92.9%～95.5%and 92.9%～104.2%.The inter-day RSD was 2.2%～9.5%,1.8%～9.8%and 2.6%～9.4%.The within-day RSD was 2.9%～9.2%,3.3%～9.6%and 3.4%～9.9%.Conclusion:The assay was simple,and sensitive.It is suitable to be used for TDM of Phenobarbital,Phenytoine and Carbamazepinum in clinical.
出处
《华西医学》
CAS
2004年第1期104-105,共2页
West China Medical Journal
