摘要
目的 :建立 HPL C法测定抗癫药苯巴比妥 (PB)、苯妥英 (PT)、卡马西平 (CBZ)的血药浓度。方法 :反相柱 ODS- Hy-persil(4.6 m m× 10 0 mm ,5︼m ) ,流动相为甲醇∶水 (5 2∶ 48) ,流速 0 .8m l/ m in,紫外检测波长 2 5 4nm ,以上 3药互为内标。标本经 CH2 Cl2 提取 ,蒸干后用流动相重溶进样。结果 :PB、PT、CBZ的保留时间分别为 3.2 2、5 .5 7、6 .80 m in;最低检测浓度分别为 0 .2 5、0 .5、0 .0 5︼g/ m l;线性范围分别为 2 .5～ 40、2 .5～ 40、1.2 5～ 2 0︼g/ ml;相对回收率分别为 10 1.49%、10 4.19%、98.70 % ;日内 RSD分别为 1.81%、5 .94%、1.81% ;日间 RSD分别为 6 .0 6 %、3.35 %、3.96 %。结论 :本法具快速、灵敏、实用等优点。
AIM: To develop a method for determination of phenobarbital (PB), phenytoin (PT), carbamazepine (CBZ) in serum by high performance liquid chromatography (HPLC) for providing a scientific basis for clinical use of antiepileptic drugs. METHODS: 0 25 ml serum sample was extracted by 2 ml CH 2Cl 2, dissolved by mobile phase. ODS Hypersil (4 6 mm×100 mm, 5 μm) column was used; mobile phase: MeOH H 2O (52∶48), flow rate 0 8 ml/min. Ultraviolet detection wavelength was 254 nm. PB, PT and CBZ were complete internal standard each other. RESULTS: The parameters of PB PT and CBZ were as follows: t R 3 22, 5 57, 6 80 min; the lowest detection concentration 0 25, 0 5, 0 05 μg/ml respectively. The linear relation was in the range of 2 5-4 0, 2 5-40 and 1 25-20 μg/ml. Relative extraction recovery rate were 101 49%, 104 19% and 98 70%. The intra day precision were 1 81%, 5 94%, 1 81% respectively. The inter day precision were 6 06%, 3 35%, 3 96% respectively. CONCLUSION: The method is rapid, sensitive and practical.
出处
《中国临床药学杂志》
CAS
2001年第1期21-24,共4页
Chinese Journal of Clinical Pharmacy
关键词
高效液相色谱
苯巴比妥
苯妥英
卡马西平
血药浓度
high performance liquid chromatography
phenobarbital
phenytoin
carbamazepine
serum drug concentration
