摘要
本文报道用反相高效液相色谱法测定４８４例服用卡马西平、苯妥英和苯巴比妥的癫痫病人的血清药物浓度。采用ＵｌｔｒａｓｐｈｅｉｅＯＤＳ（２５０×４．６ｍｍ，５μｍ）色谱柱，０．０１ｍｏｌ／Ｌ磷酸盐缓冲液（ＰＨ２．５）／甲醇（４５：５５）为流动相，洗脱经乙酸乙酯提取的血清样品，在２５４ｎｍ处检测药物和内标的峰高，以内标法定量，并对方法的回收率、准确度和精密度进行评价。血药浓度监测结果提示：三种药物在剂量不变时，浓度均波动较大，故动态监测抗癫痫药物的血药浓度对临床合理用药具有重要意义。
The method of RPHPLC was reported for monitoring simultaneousiy serum concentration of carbamaepixie, phenytoin and phenobarbitono in 484 patients. The assay consists of a single extraction of drug and internal standard from serum into othyl acetate and evaporation of organic layer. The residue is reconstituted in mobile phase and injected onto Ultrasphere ODS column, and monitored at 254 nm. The mobile phase is a mixture of methanol-0.01 mol/L phosphate buffer(pH 2.5) (56 : 45) . The limit of quantitaion for carbamiazepine is 2.65 umol/L, and phenytoin is 6.08 umol/L. The extraction recoveries are 83-97% for three drug. Within-day CVs are 0.65-3.15%. Day to day CVs are 1.56-3.69%. The relationship between drug dosage, serum Css and effective therapeutlc range were evaluated. Therapeutic drug monitoring(TDM) appears to be important clinically in guiding effective and safe usage of these deug.
出处
《华西医学》
CAS
北大核心
1994年第4期408-413,共6页
West China Medical Journal
关键词
高效液相色谱
癫痫
血药浓度
卡马西平
苯妥英
RPHPLC method
Drugn concentration in Serum
Carbamazepine
ph enytoin
Phenobarbitone.
