摘要
建立了一种直接进样HPLC法测定血清中苯妥因的方法。采用混合型苯基浸透限进柱（CAPCELL PAK MF Ph．1，20mm×4．6mm i．d．，5μm），以乙腈-磷酸盐缓冲液（pH6．9，50mmol／L，体积比5：100）为流动相，流速1．0mL／min，210nm紫外检测。苯妥因在1．0～40mg／L间线性良好（r=0．9998），日内、日间相对标准偏差（RSD）为3．6％～6．0％。检出限为0．3mg／L，回收率在98％～104％。该方法不需样品预处理，血清样品用量少，快速简便，适用于临床治疗药物监测。
A direct injection high-performance liquid chromatographh method was described for the determination of phenytoin in human serum. The HPLC column （CAPCELL PAK MF Ph-1,20 mm× 4.6 mm i. d. , 5μm） has restricted access material consisting of mixed functional groups covalently bound to silica beads. The method used acetonitrile -phosphate buffer （pH 6.9, 50 retool/L） （5 ： 100, by volume） as mobile phase with a flow-rate of 1.0 mL/min and UV detection at 210 nm. The linear range of calibration curve for phenytoin was 1.0 - 40 mg/L with a correlation coefficient of 0. 999 8. The intra- and inter-day variations were between 3.6% and 6.0%. The limit of detection was 0. 3 mg/L. The recoveries were 98% - 104% . The method can be applied to determine phenytoin concentration in clinical serum sample as it was rapid, convenient and reliable.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第8期901-903,共3页
Journal of Instrumental Analysis
