摘要
目的 建立反相高效液相色谱法测定人血浆中多奈哌齐浓度。方法 采用美国Waters公司 680型高效液相色谱仪 ,486紫外可变波长检测器 ,YMC PackODS(1 50mm× 3 .9mm ,5μm)色谱柱 ,柱温 62℃ ,以CH3OH 0 .0 1mol·L- 1 NH4 H2 PO4= 65∶35 (pH 7.50 )为流动相 ,甲泼尼龙做内标 ,在 31 4nm波长处检测。 结果 标准曲线线性范围 1 .0 0～ 2 0 .0 0 μg·L- 1 ;萃取回收率 71 .6 %～ 76 .0 % ;加样回收率 99.8%～ 1 0 8.9% ;日内RSD为 2 .7%～ 1 0 .3 % ,日间RSD为 1 .8%～ 9.9%。结论 本方法快速、灵敏、准确、简便 ,可用于多奈哌齐血药浓度测定和药动学及生物利用度研究。
OBJECTIVE: To develop a method for the determination of donepezil in human plasma by RP-HPLC. METHODS: The plasma sample was extracted with 5 mL chloroform(CHCl3), then separation was achieved on the YMC-Pack ODS(150 mm × 3.9 mm, 5 μm) column. The mobile phase was consisted of CH3OH-0.01 mol·L-1 NH4H2PO4=65:35 (pH 7. 50) and the eluate was measured at UV 314 nm. Methylprednisolone was used as the internal standard. RESULTS: The retention times for Donepezil and methylprednisolone were 7. 89 min and 6. 66 min respectively. The calibration curves were linear over the range of 1. 00-20. 00 μg·L-1. The limit of detection was 1. 0 μg·L-1. The extraction recovery of donepezil were 71. 6%-76. 0%. The recoveries were 99. 8%-108.9%. The inter-day RSD was 1. 8%-9. 9% and within-day RSD 2. 7%-10. 3%. CONCLUSION: The assay was simple, rapid, and sensitive. It is suitable to be used to study the pharmacokinetics and relative bioavailability of donepezil.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第2期137-139,共3页
Chinese Pharmaceutical Journal
关键词
反相高效液相色谱法
多奈哌齐
血药浓度
Assays
High performance liquid chromatography
Ketones
Phase composition
Sensitivity analysis
