摘要
目的 观察和比较宁夏枸杞和富硒枸杞对衰老小鼠肾脏的组织形态学影响。方法 采用D 半乳糖皮下注射法制作衰老小鼠模型 ,同时宁夏枸杞组和富硒枸杞组分别以 10 %水煎剂连续灌胃。采用光镜 ,透射电镜 ,进行组织化学观察 ,用BI2 0 0 0图像分析系统进行形态定量分析。结果 衰老模型组肾小球硬化率、肾小囊宽度、肾小球直径和面积 ,基底膜厚度明显高于对照组、宁夏枸杞组和富硒枸杞组 (P <0 .0 1或 0 .0 5 ) ;肾小球内毛细血管袢面积与肾小球面积百分比 ,肾小管周围毛细血管数量 ,裂孔隙宽度显著低于对照组、宁夏枸杞组和富硒枸杞组 (P <0 .0 1)。富硒枸杞组与宁夏枸杞组在肾小球硬化率 ,管周毛细血管数 ,基底膜厚度等指标有显著性差异 (P <0 .0 5 )。结论 富硒枸杞能改善衰老肾脏形态结构 ,起到延缓肾脏老化的作用 。
Objective To observe and compare the effect of Lycium barbarum L (LBL)and Seleniferous Lycium Barbarum L(SLBL) on the morphology of renal senility. Methods The model of senile mouse was made by subcutaneous injecting D-galactose and orall administration of 10% solution of chinese herbal medicine. Light microscopy and transmition electromicroscope were used and BI2000 image analysis system was employed for morphometric study. Results Glomerulus sclerosis rate,renal capsule breadth,renal corpuscle diameter and area,basement membrane thickness in model group were higher than those in normal control, LBL and SLBL groups (P<0.01 or 0.05). The percentage of the area of capillary lumen with that of glomerulus, the number of peritubular capillary, slit pore width in model group were lower than those in normal control, LBL and SLBL groups (P<0.01). The parameters of glomerulus sclerosis rate, the number of peritubular capillary, basement membrane thickness were different between LBL and SLBL groups(P<0.05).Conclusion SLBL was beneficial for morphology of senile kidney and has stronger delayed effect on renal senility of mouse than LBL.
出处
《解剖科学进展》
CAS
2004年第1期47-49,53,共4页
Progress of Anatomical Sciences
关键词
宁夏
枸杞
富硒枸杞
衰老肾脏
形态学
定量分析
Lycium barbarum L
Seleniferous Lycium barbarum L
kidney
senility
morphometric quantitative analysis
