摘要
采用苯酚-硫酸法衍生,紫外-可见分光光度法测定枸杞子提取物中枸杞子多糖含量。对样品制备及含量测定方法进行充分验证,最大吸收波长490nm,葡萄糖线性范围为2.65～13.3μg/mL,r=0.99939,平均回收率为101.32%,RSD为2.39%。所建立方法准确、可靠,可用于枸杞子提取物中多糖的含量测定。
Contents of Lycium barbarum L. polysaccharides in extract of lycii fructus were determined by using phenol-sulfuric acid method and ultraviolet and visible spectrophotometry.The methods of sample preparation and assay were validated.The maximum absorbance wavelenghth is 490 nm.The linearity range of saccharide content was 2.65-13.3g/mL. The linear correlation coefficient was 0.9994. The average recovery was 101.32%. RSD was 2.39%. The method is simple and accurate for determination of polysaccharides in extract of lycii fructus.
出处
《现代仪器》
2013年第1期70-72,66,共4页
Modern Instruments
关键词
紫外-可见分光光度法
苯酚-硫酸法
枸杞子提取物
多糖
Ultraviolet and visible spectphotometry Phenot-sule acid Extract oflycii ctus Polysaccharides assay
