摘要
目的 :研究 Bcl- 2、 Bax蛋白在幽门螺杆菌 (Hp)感染的慢性胃炎中的表达 ,探讨 Hp感染与细胞凋亡的关系。方法 :通过内镜活检、组织病理学检查、尿素酶试验及 Warthin- Starry银染色 ,诊断慢性浅表性胃炎 (CSG) 117例。采用免疫组化法同步检测胃粘膜 Bcl- 2、Bax蛋白的表达。结果 :Bcl- 2在 Hp阳性表达的阳性率为 2 4.5 % ,与 Hp阴性组比较无显著性差异(P >0 .0 5 ) ;在 Hp阳性轻、中、重度组的阳性率分别为 2 0 .0 %、 2 8.6 %、 2 3.5 % ,与 Hp阴性各型 CSG组亦无显著性差异(P >0 .0 5 )。Bax在 Hp阳性组中表达的阳性率为 81.1% ,在 Hp阳性轻、中、重度组的阳性率分别为 80 .0 %、81.0 %、82 .4% ,显著高于相同病变中 Hp阴性组 (P <0 .0 5 )。在 Hp感染的 CSG中 ,Bcl- 2与 Bax呈反向表达。结论 :Hp阳性 CSG各组 Bcl-2蛋白的低表达和 Bax蛋白的高表达提示这两种蛋白参与了 Hp感染胃粘膜上皮细胞凋亡的调控。
Objective:To study the expression of Bcl 2 and Bax protein in Helicobacter pylori (H pylori) infected chronic gastritis and explore the relationship between H pylori infection and apoptosis Methods: Bcl 2 and Bax protein of gastric membrance specimen in 117 patients of chronic superficial gastritis(CSG) diagnosed by endoscopy and histopathologic examination were detected by immunohistochemistry Results: The positive expression of Bcl 2 protein in H pylori positive CSG was 24 5%, which had no difference compared to the H pylori negative group(P>0 05) and the positive expression in H pylori positive mild,midium and severe CSG were 20 0%,28 6%,23 5% respectively There was no difference compared to the H pylori negative groups(P>0 05) The positive rate of Bax protein in H pylori positive CSG was 81 1%,it was significantly higher than that in H pylori negative CSG (P<0 05) The positive expression of Bax in H pylori positive mild,midium and severe CSG were 80 0%,81 0%,82 4% respectively,which were higher than that in H pylori negative groups respectively(P<0 05) The expression of both Bcl 2 and Bax protein were reverse in H pylori infected CSG Conclusion: Low expression of Bcl 2 and high expression of Bax protein in H pylori positive CSG indicate that the two proteins modulate the apoptosis in H pylori infected gastric epithelium
出处
《广西医科大学学报》
CAS
2001年第1期24-26,共3页
Journal of Guangxi Medical University
