摘要
目的 探讨Bcl 2、Bax蛋白在桥本甲状腺炎 (HT)甲状腺组织的表达及其与HT发病机制的关系。方法 采用免疫组化S P法检测 4 1例HT及 10例正常甲状腺组织Bcl 2、Bax表达及分布 ,应用Mias99图像分析系统进行定量分析。结果 (1)HT组甲状腺组织Bcl 2、Bax阳性颗粒面积、平均光密度和积分光密度均显著高于正常组 (P <0 0 1) ,HT组中组织结构破坏较重区域中小滤泡及失去正常滤泡结构的腺上皮Bax表达高于其它腺上皮 ,滤泡结构较完整的区域Bcl 2表达较高 ;(2 )HT甲状腺组织滤泡区Bcl 2阳性颗粒面积、积分光密度与Bax的比值低于正常对照组 (P <0 0 0 1)。结论 HT甲状腺组织Bcl 2、Bax表达失衡导致的细胞凋亡增高可能是HT甲状腺细胞破坏的重要机制之一 ;Bcl
Objective To investigate the relationship between the expression of Bcl 2,Bax and the pathological changes in Hashimotos thyroiditis(HT).Methods 41 cases of HT thyroid and 10 cases of normal thyroid specimens were selected.The expression and distribution of Bcl 2 and Bax was detected by using immunohistochemical methods and analyzed by Mias99 pathological image system.Immunohistochemical staining was carried out using S P kit.Results The positive granule area,average light density and total light density of Bcl 2 and Bax in thyriod tissue from HT were higher than those from normal controls( P< 0 01),The thyrocyte in disrupted follicles had an increased Bax expression.The strongly positive Bcl 2 staining was observed in the thyrocyte of intact follicle;The ratio of positive area and total light density of Bcl 1 to Bax in HT thyroid follicle area was lower than in normal thyroid.Conclusion The lost of Bcl 2 and Bax equilibrium may contribute to the pathogenesis of HT.Bcl 2 may be related to the survival of some thyrocyte in HT.
出处
《江苏医药》
CAS
CSCD
北大核心
2002年第9期653-655,共3页
Jiangsu Medical Journal
