摘要
目的：探讨幽门螺杆菌ＨＰ引起胃粘膜上皮细胞凋亡和增殖改变及ＨＰ致癌作用的可能机制。方法：采用免疫组织化学方法及脱氧核糖核酸末端转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）技术，对ＨＰ阳性和ＨＰ阴性者胃粘膜上皮中增殖细胞，凋亡细胞进行原位观察和比较，同时检测 ｂｃ１－２／ｂａｘ蛋白表达状态。结果： ＨＰ阳性者胃粘膜上皮细胞增殖指数和凋亡指数显著高于ＨＰ阴性者（Ｐ＜０．０１）。ｂａｘ蛋白表达阳性率在ＨＰ阳性组较ＨＰ阴性组升高显著（ Ｐ＜ ０． ０１），而 ｂｃｌ－２蛋白表达阳性率两组差异不显著（ Ｐ＞ ０． ０５）。在 ＨＰ阳性慢性胃炎中， ｂｃｌ－２蛋白表达阳性组细胞凋亡指数显著低于阴性组（Ｐ＜０．０１）。ｂａｘ蛋白表达阳性组细胞凋亡指数显著高于阴性组（Ｐ＜０．０１）。结论：ＨＰ可诱导胃粘膜上皮细胞凋亡和过度增殖，ｂｃｌ－２蛋白低表达和ｂａｘ蛋白高表达参与此调节机制，推测认为ＨＰ在胃癌的发生过程中可能有重要意义。
Objective: To investigate the bcl-2 and bax protein expression and its association with apoptosis and cell proliferation in Helicobacter pylori(HP) related gastritis. Methods: Using immunohistochemical staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) technique, we observed bcl-2 and bax protein expression and the change of gastric epithelial apoptotic index (AI) and proliferative index (PI) in HP-related gastritis (n=43 ). Results: AI and PI in HP positive gastritis were significantly higher than that in HP negative gastritis (P < 0. 01). The positive rate of bax protein expression in HP positive gastritis was significantly higher than that in HP negative gastritis (P < 0. 01 ), whereas the positive rate of bcl-2 protein expression was similar in both groups (P > 0.05 ). In HP positive gasitritis, the percentage of apoptotic cells in the bcl-2 negative group was higher than that in the bcl-2 positive group, the percentage of apoptotic cells in the bax negative group was lower than that in the bax positive group (P < 0. 01 ). Conclusions: HP may be involved in the induction of the apoptosis and proliferation of gastric epithelial cells. The bcl-2 may inhibit apoptosis and the bax may promote apoptosis in HP positive gastritis.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第1期40-41,53,共3页
Chinese Journal of Cancer
关键词
胃炎
细胞凋亡
PCNA
螺杆菌幽门
Gastritis
Helicobacter pylori
Apoptosis
Cell proliferation
Gene expression
