摘要
目的 :观察大鼠溃疡性结肠炎淋巴细胞凋亡及其调控蛋白bcl 2和Bax的表达及大蒜素对其的影响 ,探讨大蒜素对溃疡性结肠炎肠黏膜的保护作用及其机制。方法 :SD大鼠 48只随机分为对照组、三硝基苯磺酸组 (TNBS组 )、三硝基苯磺酸 + 0 .9%氯化钠注射液组 (TNBS +NS组 )、三硝基苯磺酸 +大蒜素组 (TNBS +Alc组 )共 4组 ,每组 12只。用TNBS 15 0mg·kg 1 灌肠建立大鼠溃疡性结肠炎模型 ,第 2天开始 ,TNBS +NS组和TNBS +Alc组分别以 0 .9%氯化钠注射液 4mL·d 1 、大蒜素 3 0mg·kg 1 ·d 1 对其进行灌胃 ,qd ,至 3周后处死动物。利用DNA缺口末端标记技术 (TUNEL法 )和bcl 2、Bax蛋白免疫组化染色 ,分别检测溃疡性结肠炎大鼠肠组织中的淋巴细胞凋亡和淋巴细胞bcl 2和Bax的表达 ,并观察肠管大体形态和组织学改变。结果 :与TNBS组及TNBS +NS组相比 ,TNBS +Alc组中淋巴细胞凋亡增加 (P <0 .0 1) ,bcl 2表达阳性淋巴细胞减少 (P <0 .0 1)。损伤指数明显下降 (P <0 .0 1)。结论 :大蒜素可以通过促进淋巴细胞凋亡而对TNBS诱导的溃疡性结肠炎大鼠肠黏膜有保护作用。
Objective:To survey the effect of allicin on apoptosis of the lymphocyte and the expression of its regulatory proteins bc1 2 and Bax in an experimental rat model of ulcerative colitis and to probe into the protective action of allicin on the intestinal mucosa of the animal as well as its underlying mechanisms. Methods:48 SD rats were randomly divided into 4 equal groups, with 12 animals in each group: group(1): blank control; group(2): TNBS (trinitrobenzenesulfonic acid) treated; group(3): TNBC+0.9% NaCl solution; group(4): TNBS+allicin. The rat model of ulcerative colitis was brought about by an enema of 150 mg·kg 1 of TNBS. Beginning from the 2 nd day, gastrogavage was used to administer 4 ml of 0.9% NaCl solution to each animal of group3 (TNBS+NS) and 30 mg·kg 1 of allicin to each rodent of group(4) (TNBS+allicin)q.d.. The rats were sacrificed 3 weeks later. Apoptosis of the lymphocyte was detected with the technique of TUNEL (Tdt mediated dUTP nick end labeling) while bcl 2 and Bax protein expressions were assayed with immunohistochemical staining. Besides, the macroscopic and histologic changes in the intestinal tract were also kept under observation. Results:As compared with changes in animals of group(2) (TNBS treated) and group(3) (TNBS+NS), apoptosis of lymphocytes was increased( P <0.01) while lymphocytes expressing bcl 2 protein were decreased ( P <0.01) in animals of group(4)(TNBS+allicin). The index of injury in this group was significantly decreased( P <0.01). Conclusion:Allicin was shown to exert a protective effect on the intestinal mucosa in a rat model of ulcerative colitis possibly by promoting apoptosis of lymphocytes in lamina propria.
出处
《医药导报》
CAS
2003年第12期835-838,共4页
Herald of Medicine
基金
湖北省教育厅重点基金资助项目 (基金编号 :2 0 0 2A0 40 0 6)
