摘要
以聚乙烯吡咯烷酮(PVP)为添加剂对毛细管柱进行动态修饰,用于碱性蛋白分离。对4种碱性蛋白质进行分离,实验结果表明聚乙烯吡咯烷酮能够很好地抑制电渗流(EOF)及碱性蛋白在石英毛细管壁上的吸附作用。在pH4.0,PVP浓度(W/V)为0.4％时,EOF仅为1.35×10^(-9) m^2/V.s,平均柱效可达5×10~5理论塔板数/m。每次运行之间(n=6),天与天之间(n=6),迁移时间的相对标准偏差(RSD％)分别小于1％和3％,表明该动态涂敷方法具有良好的重现性和稳定性。另外,由于PVP的粘度较小,使得该方法操作方便、快捷。
A new and simple method to diminish the adsorption of basic proteins in capillary electrophoresis with untreated silica capillary is described. In this method, small amount of poly (vinylpyrrolidone) (PVP) is used as a buffer additive for dynamically modifying the capillary wall. It is shown that PVP can effectively suppress the electroosmotic flow and prevent adsorption of cationic proteins such as cytochrome C, lysozyme , trysinogen , and α-chymotrypsinogen A. The average theoretical plate per meter of the four basic proteins is more than 5×10~5 in a pH 4.0 buffer containing 0.4% PVP ( WI V) . Migration time reproducibility for those proteins is < 1 % RSD within 1 day (for continuous 6 runs) and < 3% from day to day (6 days). These represent good reproducibility and stability of the dynamic coat. The another advantage is that column regeneration between runs is very simple and fast due to the low viscosity of PVP.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第5期585-588,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题(29875033)
关键词
PVP
毛细管电泳
碱性蛋白质
分离
蛋白质
Dynamically coated column, poly ( vinylpyrrolidone), capillary electrophoresis, basic proteins
