摘要
建立了一种简便的制备交联聚丙烯酰胺型毛细管电泳涂层柱的方法.所制备的涂层柱能够有效地抑制电渗流及蛋白质在管壁上的吸附.考察了碱性蛋白质在pH=4.0的缓冲溶液中的分离,迁移时间重现性误差小于2.1%.对麻黄提取物中的生物碱进行了分离,平均理论塔板数为2.4×105p lates/m.
A method was developed for the preparation of static coating onto the fused-silica columns with cross-linked polyacrylamide. The coated columns were effective to suppress the electroosmotic flow and prevent the absorption of basic proteins on the column wall. The basic proteins were separated by using the coated columns at pH = 4.0, with retention time variation of less than 2.1% RSD. The average separation efficiencies were 2.4 ×10^5 plates/m for alkaloids. The cross-linked polyacrylamide coated columns can be prepared reproducibly by using this method.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第2期218-221,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20227501)资助
关键词
交联聚丙烯酰胺涂层柱
毛细管电泳
蛋白分离
生物碱分离
Cross-liked polyacrylamide-coated column
Capillary electrophoresis
Protein separation
Alkaloid separation
