摘要
针对常用乳糖测定方法存在的局限性和不便性,结合高效毛细管电泳(HPCE)法简单快速、高效低耗的特点,探索出了高效毛细管电泳仪配合紫外检测器测定乳糖的实验条件:熔融石英毛细管(50μm×34 cm);缓冲液为50 mmol/L硼砂/NaOH,pH值为10.0;电泳为10 kV,温度为60℃;检测波长为195 nm;进样为5000 Pa;时间3 s。此方法操作简便、准确度高、精密度好。该方法的测定结果与高效液相色谱法的结果较一致,样品中其他糖类对该方法不存在干扰,克服了莱因-埃农氏法测定乳糖不具专一性的缺点。
The disadvantage of present usual methods of determination of lactose is their limitations or inconveniences. In this paper, the optimal experimental conditions of determination of lactose in milk by high-performance capillary electrophoresis （HPCE, Agilent, HP3DG1601BA ） with UV detector were explored： fused silica capillary （50℃m ID×34 cm）, buffer solution： 50 mM borate / NaOH, pH 10.0, HPCE conditions： 10 kV, 60 ℃, UV detection at 195 nm, sample was injected at 5 000 Pa for 3 s. This method is easy to operate and has good accuracy and precision. The result of this method is consistent with high-performance liquid chromatography （HPLC） method when they detect the lactose in milk, and other reducing sugars in milk will not interference the result of HPCE method.
出处
《中国乳品工业》
CAS
北大核心
2011年第10期44-47,共4页
China Dairy Industry
关键词
液态乳
乳糖
高效毛细管电泳
milk
lactose
high-performance capillary electrophoresis
