摘要
目的 建立黄毛耳草中槲皮素含量的测定方法。方法 色谱柱:ODSKromasil C18色谱柱(4.6mm×150mm,5μm);柱温:35℃;流动相:甲醇-0.4%磷酸(体积比45:55),流速:1.0mL/min,检测波长:365nm。结果 槲皮素进样量在0.005—0.045μg范围内与峰面积线性关系良好(r=0.9997),回收率99.88%,RSD 0.58%。结论 本法简便,结果可靠,可用于黄毛耳草厦其制剂的质量控制。
Objective To establish an HPLC method for content determination of quercetin in Hedyotis chrysoticha ( Palib. ) Merr. Methods HPLC method was carried on Kromasil C18 column using methanol-0.4% phosphoric acid (45:55 ) as the mobile phase under the detection wavelength of 365 nm. Results The calibration curve for quereetin was linear in the range of 0. 005 - 0. 045μg. The average recovery was 99.88 % with RSD 0. 58 %. Conclusions The method is simple, convenient and reliable for quality control of Hedyotis chrysoticha (Palib.) Merr.
出处
《广东药学院学报》
CAS
2006年第1期57-58,共2页
Academic Journal of Guangdong College of Pharmacy