摘要
【目的】建立鹿根药材中槲皮素HPLC含量测定的方法。【方法】以槲皮素为对照品,采用高效液相色谱法,对鹿根药材中槲皮素含量进行测定。流动相：乙腈-1%冰醋酸,色谱柱为Waters XTerra RP C18（250 mm×4.6 mm,5μm）色谱柱,流速：1.0 mL/min,检测波长：360 nm,柱温：25℃。【结果】槲皮素在0.086 60.866μg范围内呈良好的线性关系（r=0.999 9）,加样回收率为100.68%,RSD为1.18%。【结论】该方法简便、准确、灵敏度高、重现性好,能有效测定鹿根药材中槲皮素含量,可为鹿根药材的质量评价提供科学依据。
【Objective】To set up a method for determining the quercetin in Rhaponticum carthamoides（Willd.） Iljin.【Method】Quercetin was used as a reference substance in this method.The quality determination of quercetin in Rhaponticum carthamoides（Willd.） Iljin was determined by HPLC on Waters XTerra RP C18（250 mm×4.6 mm,5 μm） with acetonitrile-1% acetic acid as the mobile phase.The flow rate was 1.0mL/min,the detection wavelength was 360 nm and the column temperature was 25℃.【Result】The calibration curves of quercetin was linear within the range of 0.086 60.86 6 μg,the correlation was 0.999 9,and the average recovery was 100.68%（RSD=1.18%）.【Conclusion】This method is simple,accurate highly sensitive and reproducible.It could be used for the quality determination of quercetin in Rhaponticum carthamoides（Willd.） Iljin.It could be used as scientific reference to evaluating the quality of Rhaponticum carthamoides（Willd.） Iljin.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2010年第11期2329-2332,共4页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区青年自然科学基金项目(2009211B39)
关键词
鹿根
槲皮素
含量测定
HPLC
Rhaponticum carthamoides （ Willd. ）lljin
Quercetin
Determination
HPLC
