摘要
目的:采用“醇提凝析法”分离制备黄毛耳草中的乌索酸,建立其含量的测定方法 方法:IR、MS、1HNMR、13CNMR鉴定乌索酸结构,反相高效液相色谱法测定其含量,色谱柱为SymmetryShieldRP1 8柱(3 . 9×1 5 0mm ,5 μm) ;流动相为甲醇∶水(88∶1 2 ,V/V) ;流速为1 0mL/min ;二极管阵列检测器检测,检测波长2 1 0nm ;柱温2 5℃;结果:确证化合物为乌索酸 HPLC法线性范围:4 . 5～2 2 . 5 μg ;线性回归系数大于0 . 9998 乌索酸纯度为99 . 82 %,得率3 . 6 5‰ 结论:制备方法实用可行,乌索酸纯度高,安全性好 HPLC法快速简便、精密度高、重现性好、线性范围宽。
Objective: To separate and extract the Ursolic acid from Hedyotis corymbosa Lam by adopting the method of 'ethanol extracting and agglutination' and to determine the content of ursolic acid . Method: Using spectrum to identify the structure of ursolic acid by IR, MS,1HNMR 13CNMR. Adetermination method of ursolic acid by reversed-phase high performance liquid chromtograph was established. The chromatographic column, SymmetryShield RP18 (3.9×150 mm,5 μm), methanol-water (88:12, V/V) mobile phase with 1.0 mL/min flow rate, the detected wavelength (210 nm), and the column temperature(25 ℃) were adopted. Result: The compound is recognized to be ursolic acid. The calibration curve of ursolic acid by HPLC was linear under the content of 4.5～22.5 μg, the correlation coefficient was over 0.9998. The content is 99.82% and the gain rate is 3.65‰. Conclusion: The preparation method is practical and feasible. The purity of ursolic acid is high and the safety is good. The analytical method is simple, accurate and has good repeatability and wider linear range. It can be adapted to evaluate the quality of ursolic acid.
出处
《宜春学院学报》
2005年第2期1-4,共4页
Journal of Yichun University
基金
国家"8 63"计划重点资助项目 (2 0 0 2AA2Z3 2 17)
