摘要
基于亚硝酸根对溶解氧氧化溴连苯三酚红的催化作用，建立了双波长分光光度法动力学测定亚硝酸根的新方法。于３９２ｎｍ处测量体系吸光度Ａ＿１，４８４ｎｍ处测量体系吸光度Ａ＿２，以ΔＡ＝Ａ＿１－Ａ＿２进行亚硝酸根定量，所建立的方法对亚硝酸根的线性范围为０．００５～１．０μｇ／ｍＬ。该法简便、灵敏、选择性好、应用于蔬菜样品的测定，结果满意。
dual-wavelength kinetic spectropho-tometric method for the determination of ni-trite is described ,based on its catalytic effecton the reaction between dissolved oxygenand bromopyrogallol red in acidic medium.The reaction is monitored spectropho-tometrically by measuring the absorbance in-crease at 392nm(A_1) and the absorbance de-crease at 484nm(A_2). Nitrite is determiriedaccording to△A=A_1-A_2=A_1+|A_2|.Thelinear range for nitrite is 0. 005～1.0μg/mL.the method is of high sensitivity,selectivity,accuracy and simplicity,The proposedmethod has been successfully applied to thedetermination of nitrite in some vegetablesamples.
出处
《分析试验室》
CAS
CSCD
北大核心
1996年第2期56-58,共3页
Chinese Journal of Analysis Laboratory
关键词
光度法
亚硝酸根
溴连苯三酚红
蔬菜
inetic spectrophotometry
bromopyrogallol red
nitrite determination
