摘要
研究了十二烷基苯磺酸钠 (SDBS)存在下氯化血红素 (Hemin)类似过氧化物酶的新型催化活性。在 pH 8 0～ 8 9氨性缓冲介质中 ,Hemin催化溶解氧氧化溴邻苯三酚红的褪色反应。在SDBS和 30 %乙醇的增效作用下 ,催化体系服从Michaelis Menten方程。用Lineweaver Burk作图法求得米氏常数、催化常数和专一性常数分别为 3 7× 10 - 5mol/L ,0 6 4s- 1和 1 7× 10 4 L·mol- 1·s- 1。对机理进行了讨论。利用该催化体系 ,用反应平衡法和动力学法均可分光光度定量Hemin ,线性范围为 2 0× 10 - 8～ 6 0× 10 - 7mol/L ,检测限为 2 5× 10 -
A novel catalytic property of hemin is described.In ammonium buffer medium of pH 8 0～8 9,hemin has an ability to catalyze the decoloration of bromopyro\|gallol red.Oxygen dissolved in solution plays a role as an oxidant in the reaction.With sodium dodecylbenzenesulfonate and ethanol as effect\|enhancing agents,the catalytic system obeys the Michaelis\|Menten equation of enzymatic catalysis.The Michaelis constant,catalytic constant and specificity constant of the system are estimated by Lineweaver\|Burk plot to be 3.7×10 -5 ?mol/L,0.64?s -1 and 1.7×10 4?L·mol -1 ·s -1 ,respectively.The mechanism of the hemin oxidase\|like catalytic system is discussed.Based on this,hemin can be spectrophotometrically determined by equilibrium and kinetic procedures with a linear range and 3 σ limit of detection being 2.0×10 -8 ～6.0×10 -7 and 5.0×10 -9 ?mol/L,respectively.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第2期161-166,共6页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
国家自然科学基金项目! (2 9875 0 11)
教育部跨世纪优秀人才基金项目
中国博士后科学基金项目
