摘要
苜蓿根瘤菌ＡＳ１．１５９经亚硝基胍和Ｃｏ￣（６０）γ射线诱变，获得一株高积累聚β－羟基丁酸（ＰＨ８）突变株Ｊ５１７７，积累的ＰＨＢ可达细胞干重的３０％，约为原出发菌株的１０倍。采用分批补料发酵法，积累的ＰＨＢ可达细胞干重的７１％。菌体生长最适Ｃ／Ｎ比值为２．５，菌体积累ＰＨＢ最适Ｃ／Ｎ比值为１５；ＣａＳＯ＿４，Ｎａ＿２ＭｏＯ＿４对菌体积累ＰＨＢ有促进作用。
A mutant strain J 5177 with a high yield of poly-β-hydroxybutyric acid from Rhizobium melitoti AS 1.159 was obtained by chemical mutation using N-methyl-N'-nitro-soguanidine(MNNG)and treatment with Co_(60) radiation. The content of PHB obtained in batch culture was 30%,which was about 10 times that of the originel strain’s,The optimal combination of carbon source and nitrogen source for the growth of the strain was 2.5,and PHB accumulation was 15.There was the promotion of CaSO_4 and Na2MoO_4 to PHB accumulation in batch culture. The optimal medium volume was 125 mL in a 500 mL flask,The maximum content of PHB was 71%,which was obtained by fed-batch fer-mentation providing supplementation of glucose.
出处
《吉林大学自然科学学报》
CAS
CSCD
1995年第2期98-100,共3页
Acta Scientiarum Naturalium Universitatis Jilinensis
