摘要
真养产碱菌(Alcaligenes eutrophus)H16能以果糖为碳源在无机合成培养基上积累聚p-羟基丁酸(PHB)。将该菌用富含果糖的高果糖浆(HFS)培养,PHB的积累可达到果糖发酵水平,但发现高浓度的果糖和葡萄糖对菌体生长及PHB积累有抑制作用。采用补料分批培养技术可降低果糖和葡萄糖的抑制。并可大幅度提高产量,菌体干重达16—20g/L,PHB产量7.0—7.6g/L,PHB的产率达0.24g/g果糖。
Fructose can be utilized by A. eutrophus to accumulate poly-β-hydroxybutyric acid (PHB) in mineral medium. When fructose was replaced by HFS, the accumulation of PHB from HFS amounted to those of PHB from fructose. There existed the inhibition of high fructose and glucose concentration on cell growth and PHB accumulation. The fed-batch culture can eliminate the inhibition. The yield of PHB production was about 7. 0-7. 6g/L and cell biomass was about 16-20g/L.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第2期115-120,共6页
Acta Microbiologica Sinica
