摘要
研究了真养产碱杆菌突变株６５－７，以葡萄糖为主原料，添加丙酸或戊酸，采用二步发酵积累共聚物聚β－羟基丁酸－β－羟基戊酸（ＰＨＢＶ）。摇瓶总发酵时间为５０ｈ，细胞干重达７－１１ｇ／Ｌ，共聚物含量占细胞干重的７０％以上，其中β－羟基戊酸（３ＨＶ）含量占ＰＨＢＶ的１０－７２％，主要取决于不同碳源的组成，丙酸和戊酸对ＨＶ的转化率分别为０．４１－０．６３ｇＨＶ／ｇ丙酸和０．４０－０．７４ｇＨＶ／ｇ戊酸，制得的ＰＨＢＶ产品纯度９９％以上，分子量６．９×１０￣５。
By two-stage fermentation,the synthesis of the copolymer of 3-hydroxybu-tyrate(3HB) and 3-hydroxyvalerate(3HV),PHBV,by the mutant 65-7 of Alcaligeneseutrophus from glucose and propionic(or pentanoic) acid was studied.For 50 hours,thetotal cell concentration in shake-flask was 7-10g/L,and the PHBV content reached upto70%of the dry cell weight.The 3HV fraction in the copolymers was 10-74%,dependingon the composition of carbon suhstrates supplied. The convertion from propionic and penta-noic acid to 3HV was 0.41-0.63 and 0.40-0.74 wt/wt, respectively.The molecular weightof PHBV isolated from the biomass was 6.9× 10￣5 with purity more than 99%.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第5期271-275,共5页
Microbiology China
