摘要
通过对含有PHB和非PHB(脂类贮存物质(NPLD)的细菌细胞的尼罗红染色和荧光显微观察,证实尼罗红是一种很好的细菌细胞内贮存的脂类物质的荧光染色剂,灵敏度较高.可用于PHB和非PHB脂类贮藏物质荧光显微观察,并能在一定程度上将两者区分开来.
Nile red, a bcnzophenoxazone stain was attempted for microscopical observation of poly-β-hydroxybutyric acid (FHB) and other lipid deposits in bacterial cells. Two kinds of the lipids were distinct to some extent with fluorescence microscopy after Nile red staining. As a superior fluorescence stain, its potential for lipid detection in bacterial cells deserves further exploration.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第1期71-75,共5页
Acta Microbiologica Sinica
关键词
尼罗红
细菌
脂类
羟基丁酸
贮藏物
Nile red, Poly-β-hydroxybutyric acid, Lipid storage material, Bacteria
