摘要
在酸性条件下,水溶苯胺蓝(ABWS)与硫酸庆大霉素(GEN)或硫酸妥布霉素(TOB)反应,生成离子缔合物,使水溶苯胺蓝溶液褪色,其最大褪色波长分别位于606nm(GEN)和608nm(TOB);在0～1.2×10-5mol·L-1(GEN)和0～1.5×10-5mol·L-1(TOB)的浓度范围内遵从比耳定律;表观摩尔吸光系数(ε)分别为2.21×104L·mol-1·cm-1(GEN)和3.10×104(TOB)L·mol-1·cm-1。本法已用于市售药物及人体尿液中庆大霉素及妥布霉素含量的测定,结果满意。
In acid solution, aniline blue water soluble(ABWS) reacts with gentamycin sulfate(GEN) or tobramycin sulfate (TOB) to form ion association complex, which causes the fading of aniline blue water soluble. The maximum fading wavelengths were located 606 nm(GEN) and 610 nm(TOB), respectively. Beer’s law was obeyed in the range of 0~1.2×10 -5 mol·L-1 for GEN and 0~1.5×10-5 mol·L-1 for TOB, respectively. Their apparent molar absorptivities(ε) were 2.21×10 4 L·mol -1 ·cm -1 (GEN) and 3.10×104 L·mol -1 ·cm -1 (TOB),respectively. The method was used to determine the contents of gentamycin and tobramycin commercial available and in human urine with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
2005年第3期301-303,共3页
Journal of Analytical Science
关键词
水溶苯胺蓝
庆大霉素
妥布霉素
褪色光度法
Aniline blue water soluble
Gentamycin
Tobramycin
Fading spectrophotometry
