摘要
在酸性条件下,水溶苯胺蓝(ANB)与硫酸阿米卡星(AMK)反应,生成蓝色离子缔合物,其最大正吸收波长位于682nm,最大负吸收波长位于602nm,正负峰吸光度绝对值之和与AMK的浓度线性相关,AMK的浓度在0～1.8×10-5mol·L-1的范围内遵从朗伯比耳定律,表观摩尔吸光系数为9.50×104L·mol-1·cm-1,由此建立了测定阿米卡星的双波长光度法。方法用于市售药物及人体尿液中阿米卡星含量的测定,结果满意。
In a BR-buffer solution of pH 3.8, a blue ion-association complex was formed by the reaction between water soluble aniline blue (ANB) and amikacin (AMK). DW-photometric measurements were made at 602nm (λ_1 wavelength of reference) and 682nm (λ_2 wavelength of determination), and linear relationship between ΔA and concentration of amikacin was found in the range of 0～1.8×10^(-5)mol·L^(-1) of amikacin. Apparent molar absorptivity was found to be 9.5×10~4L·mol^(-1)·cm^(-1). This method has been satisfactorily use in the determination of amikacin in some market-sale drugs and in human urine.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2004年第6期338-340,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
光度法
阿米卡星
水溶苯胺蓝
双波长
DW-spectrophotometry
Amikacin
Water solube aniline blue
Drug
Human urine
