摘要
对不同外植体来源的茶树培养细胞的茶氨酸生物合成能力、适宜培养细胞合成茶氨酸的培养基条件、培养细胞茶氨酸生物合成动态和更新培养基对提高培养细胞茶氨酸累积含量的效果等进行了分析。在一个培养周期中,培养细胞的茶氨酸累积高峰出现在第11 d左右。在以每10 d更新一次培养基的条件下,培养细胞茶氨酸合成能力可维持至第30 d左右,达到细胞干重的近20%。
The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30th day and reached a high peak of 20% cell dry weight.
出处
《茶叶科学》
CAS
CSCD
北大核心
2004年第2期115-118,共4页
Journal of Tea Science
基金
浙江省自然科学基金
青年科技人才培养专项资助
