摘要
从７４７条发根农杆菌ＡＴＣＣ１５８３４转化的青蒿株系０２５发根中，筛选出７个生长较快的发根系，这７个系在生长速度和青蒿素含量上均有显著差异，其中发根系ＨＲ９青蒿素产率最高，达到每月３３２５ｍｇ／Ｌ。青蒿发根的生长量和青蒿素含量极显著高于未转化根和愈伤组织。青蒿发根在分批培养中没有明显的迟滞期，接种后第７天进入指数生长期，第１１天生长最快，第２０天进入稳定期。青蒿发根中青蒿素含量呈明显的“与生长相关”特性，在指数生长期，青蒿素含量缓慢下降，生长速度减缓后，青蒿素含量上升，发根生长停止后，继续延长培养时间，青蒿素含量也不再提高。在分批培养中，青蒿发根适宜的培养时间为２１ｄ。
Seven hairy root lines with fast growing and high artemisinin contents properties were selected from 747 hairy roots of Artemisia annua L.strain 025 transformed by Agrobacterium rhizogenes ATCC15834.The differences of growth rate and artemisinin contents among the 7 selected hairy root lines were extremely significant.In which HR 9 gave the hightest artemisinin productivity reached 33 25 mg/month·L. The differences of growth rate and artemisinin contents among hairy root,untransformed root and callus were also significant.There were no obvious lag stage in batch culture of Artemisia annua L hairy root,7～15 days after inoculation was the exponential stage,the growth rate of 11th day was the highest,and the cultures reached stationary stage at 20th day.The properties of artemisinin content of hairy root were obviously “related to growth”.The artemisinin content decreased slowly during exponential stage and increased while the growth slowed down and kept consistent after the growth stopped.The optimum culture interval of hairy root of Artemisia annual L.was 21 days.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第4期401-404,共4页
Chinese Journal of Biotechnology
基金
国家自然科学基金
关键词
青蒿
发根
发根农杆菌
青蒿素
生物合成
Artemisia annua L.hairy root, Agrobacterium rhizogenes ,artemisinin
