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海洋真菌Thraustochytrium sp.FJN-10 Δ~4脱饱和酶基因的cDNA克隆及结构分析 被引量：7

Identification of the Δ~4 fatty acid desaturase gene from Thraustochytrium sp.FJN10
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摘要 海洋真菌Thraustochytriumsp.FJN-10是二十二碳六烯酸(Docosahexaenoic acid,DHA,C22:6n-3)的高产菌株。为阐明其DHA生物合成机制及采用基因工程技术提高DHA生物合成能力,通过RT-PCR扩增了DHA生物合成相关脱饱和酶基因的保守区,采用cDNA末端扩增技术(Rapid amplification of cDNA ends,RACE)获取全长基因,结果分析表明其克隆基因为Δ4脂肪酸脱饱和酶基因,开放阅读框由1560个核苷酸组成,共编码519个氨基酸,这是Thraus-tochytriumsp.FJN-10Δ4脂肪酸脱饱和酶基因研究的首次报道。 Thraustochytrium sp. FJN10 is a marine fungi producing docosahexaenoic acid （DHA） highly. For the purpose of understanding the mechanism of biological synthesis of DHA and enhancing the biosynthesis of DHA by genetic engineering, the conserved region of delta-4 fatty acid desaturase from Thraustochytrium sp. FJN10 was amplified and sequenced by RT-PCR. Its entire ORF（open reading frame） was obtained by RACE（rapid amplification of cDNA ends）. The results showed that the full-length cDNA of delta-4 fatty acid desaturase was identified, its whole ORF was 1560 bp encoding 519 amino acids. It was the first report that the delta-4 fatty acid was isolated from the marine fungi of Thraustochytrium sp. FJN10.
作者 刘丽霞 江贤章 覃文新 姚根富 黄建忠
机构地区 福建师范大学生命科学学院 上海市肿瘤研究所
出处 《工业微生物》 CAS CSCD 北大核心 2006年第1期1-6,共6页 Industrial Microbiology
基金 国家自然科学基金项目(编号30370028) 福建省科技厅重大项目(项目编号:2003F005)
关键词 海洋真菌 二十二碳六烯酸 △^4脂肪酸脱饱和酶 RACE marine fungi docosahexaenoic acid（DHA） delta-4 fatty acid desaturase RACE
分类号 Q78 [生物学—分子生物学] Q933 [生物学—微生物学]
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参考文献24

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二级参考文献23

  • 1Mori T A, Bao D Q, Burke V, et al. Docosahexaenoic acid but not eicosapentaenoic acid lowers smbulatory blood pressure and heart rate in humans [J]. Hypertension, 1999,34:253.?A
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工业微生物
2006年 第1期

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