摘要
对不同的培养条件下茶愈伤组织生长及其茶氨酸累积情况进行了研究。结果表明，激素ＩＡＡ和６－ＢＡ结合作用时，存在着相互效应，其中以ＩＡＡ２ｍｇ／Ｌ和６－ＢＡ４ｍｇ／Ｌ时对茶氨酸累积最有利。不同碳源对于愈伤组织生长和茶氨酸积累的影响相近。增加糖浓度有利于次生代谢物的累积，茶氨酸组织的最适培养温度和茶氨酸累积温度都是２５℃。暗培养比有光培养更有利于茶氨酸的累积。茶氨酸的累积曲线与愈伤组织生长密切相关，调节培养基成分使茶氨酸在茶愈伤组织（干质量）中的含量达到了２０１．６ｍｇ／ｇ．
The growth and theanine accumulation of tea callus were investigated under different culturing conditions.Results showed that the effects of IAA and 6 BA were different when they were used together.The combination of 2mg/L of IAA and 4ml/L of 6 BA was found to be the best for the theanine accumulation.The effects of different carbon source for the growth and theanine accumulation of tea callus were almost similar.Increasing of sucrose concentration was beneficial to the accumulation of the secondry metabolism.The optimum temperature (25℃) for theanine accumulation was same as that for growth of callus.Dark culture was better than illumination for the accumulation of theanine.During a growth cycle,the accumulation of theanine was strongly dependent on the increasing of the callus fresh weight.The theanine content of the callus could be raised to 201.6mg/g weight by adjusting the medium compositions for the cultures.
