摘要
目的 :应用HPLC法进行当归指纹图谱研究。方法 :Zorbax 30 0SBC1 8分析柱 ,CH3CN H2 O H3PO4 梯度洗脱 ,流速 1ml min ,检测波长 2 80nm。结果和结论 :建立了当归的指纹图谱检测标准 ,共有指纹峰 16个 ,鉴定其中三个为阿魏酸、洋川芎内酯H、洋川芎内酯I。实验方法简便、准确、重复性好。
Objective:To study the fingerprints of Radix Angelicae Sinensis.Method:Zorbax 300SB C_ 18 column was used,with a mixture liquid of CH_3CN-H_2O-H_3PO_4 mobile phase in a gradient mode.The wavelength of measurement was 280nm.Result and conclusion:Sixteen peaks has been gotten from the Radix Angelicae Sinensis as its fingerprints.Three of them were identified as Ferulic acid,Senkyunolide H and Senkyunolide respectively.The method is simple and reliable.
出处
《中国实验方剂学杂志》
CAS
2004年第3期1-3,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家十五攻关项目 (2 0 0 1BA70 1A2 9)
关键词
当归
HPLC
指纹图谱
Radix Angelicae Sinensis
HPLC
Fingerprints
