摘要
目的建立当归挥发油中主要成分Z-藁本内酯的快速分离纯化方法,并考察其在制剂中的稳定性。方法采用中压柱色谱法和GC-MS结合计算机分离鉴定当归挥发油中的Z-藁本内酯,以色谱峰面积归一化法进行定量,并考察其在当归挥发油,Z-藁本内酯纯品,当归挥发油自乳化微乳制剂中的稳定性。结果得到了纯度大于98%的Z-藁本内酯纯品;Z-藁本内酯纯品在室温及低温下均不稳定,总油中Z-藁本内酯于室温缓慢降解,其挥发油的自乳化制剂在室温下稳定。结论分离方法适合当归中Z-藁本内酯的快速制备;当归挥发油的自乳化微乳制剂可改善其中Z-藁本内酯的不稳定性。
OBJECTIVE To establish up a method for isolation and purification of Z - ligustilide from the essential oil of Radix Angelicae Sinensis （Danggui） and to evaluate stabilities of Z- ligustilide in different formula（purified compound, total essential oil or self - nanoemulsified drug delivery systems （SNEDDS） ）. METHODS The volatile component Z - ligustilide was purified from total essential oil of Danggui by middle - pressure column chromatography and identified by GC - MS. The purity of ligustilide was Calculat- ed by area normalization. Short - term and long - term stability of ligustilide in different formula were also evaluated. RESULTS Z - ligustilide was identified by GC - MS with the purity of 98%. Short - term stability studies showed that ligustilide was not stable when being stored at 60℃ either as the pure compound or in the oil for 10 days. However, ligustilide in SNEDDS was stable to elevated tem- perature （60℃）, refrigerated condition （4℃ and -20% ） or ambient temperature. The optimized SNEDDS formulation consisted of DG oil, ethyl oleate, Tween 80, Labrasol and Transcutol. CONCLUSION The present study demonstrated a method to purify and identify Z - ligustilide from the essential oil of DG. Stability of ligustilide has been greatly improved after preparation into SNEDDS.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第5期533-535,共3页
West China Journal of Pharmaceutical Sciences
基金
澳门大学科研基金(RG072/05-06S/08T)
