摘要
目的借鉴血清药物化学的方法,探讨川芎效应组分在血液及靶组织脑脊液中的移行成分(即药物以原型进入到体液的化学成分),从而为快速、有效地确定川芎效应组分的物质基础,进一步建立其完善的质量标准奠定基础。方法通过HPLC指纹图谱和紫外光谱分析,比较空白血清、含药血清,空白脑脊液、含药脑脊液成分异同,确定川芎效应组分的血中移行成分与脑脊液移行成分,并采用HPLC-DAD-MS/MSn分析技术推测移行成分的化学结构。结果阿魏酸、洋川芎内酯Ⅰ为川芎效应组分的血清移行成分,洋川芎内酯Ⅰ为其脑脊液移行成分,即洋川芎内酯Ⅰ为川芎效应组分即入血又入脑的化学成分。结论洋川芎内酯Ⅰ很可能是川芎效应组分治疗偏头痛最重要的生物活性成分。
OBJECTIVE To study the absorbed constituents of the Chuanxiong active parts （CXAP） for the treatment of migraine following oral administration.METHODS The compound peaks of blank serum,drug-contained serum and cerebrospinal fluid were compared by HPLC fingerprints and UV spectrums.The absorbed ingredients of CXAP were structurally identified by HPLC-DAD-MS/MSn.RESULTS Following oral administration of CXAP,ferulic acid and senkyunolide I were found in serum.However,only the latter was detectable in the cerebrospinal fluid.CONCLUSION Senkyunolide I may be the most important bioactive component of Chuanxiong for the treatment of migraine.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第9期694-697,共4页
Chinese Pharmaceutical Journal
基金
上海市教育委员会科研创新项目(09YZ117)
上海市教育委员会重点学科资助项目(J50302)
关键词
川芎
效应组分
移行成分
物质基础
Chuanxiong
ative parts
migrated constituent
substance basis
