摘要
目的:建立广陈皮的HPLC指纹图谱,为其质量评价提供依据。方法:采用HPLC梯度洗脱技术进行分离分析,采用ODS-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-2%乙酸梯度洗脱,流速1.0 mL.min-1,柱温25℃,检测波长300nm,记录广陈皮与陈皮的HPLC指纹图谱。结果:广陈皮均有8个共有峰,多数的峰可以达到较好分离。普通的陈皮有8个共有峰,多数的峰可以达到较好的分离。建立的图谱有较高的重现性。从两者指纹图谱可以鉴别出广陈皮与陈皮。结论:该分析方法具有良好的精密度、稳定性和重复性,建立的指纹图谱检测标准可以作为广陈皮和陈皮质量评价的主要依据之一。
Objective： To eatablish HPLC fingerprints of Citrus nobilis var.chachikan Wong and C.reticulata Blanco and to provide a base for the identification of its quality.Method：A gradient separated method was applied.ODS-C18 column（4.6 mm × 250 mm,5 μm）,mobile phase was methanol-water,detection wavelength was at 300 nm,flow rate was 1.0 mL.min-1,column temperature was at 25 ℃.Result： Eight mutual peaks in C.nobilis var.chachikan Wong and eight mutual peaks in its preparations were separated effectively,the same as C.reticulata Blanco.The methodological evaluation showed that this method had a good repeatability.Conclusion：The results of peak areas were in accordance with the request of fingerprint.The established fingerprint can be used for the quality control and species identifying of C.nobilis var.chachikan Wong,C.reticulata Blanco and its preparations.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第7期90-93,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技计划项目(2009B030801044)
广东药学院引进人才科研基金(2007ZYX09)
关键词
广陈皮
陈皮
指纹图谱
反相高效液相
C.nobilis var.chachikan wong
Citrus reticulata Blanco
fingerprint
HPLC
