摘要
利用不同分离培养基从不同生态区域土壤中分离纤维素分解菌,针对其中木霉T1、T4与毛壳菌G2、G3菌株进行生物学特性研究,结果表明:不同菌株对营养成分变化反应不同,T1、T4、G2菌株最适天然培养基为胡萝卜煎汁与番茄煎汁,G3最适天然培养基为菠菜与马铃薯;T1、T4最适合成培养基分别为Mayer与Boas,G2、G3最适合成培养基分别为Mayer、Hopkin;T1、T4最适碳源分别为果糖与麦芽糖,G2、G3最适碳源为麦芽糖;T1、T4最适氮源为蛋白胨,G2、G3最适氮源为豆饼粉;但光照对不同菌株影响均不大;四菌株对温度反应相同,最适温度均为25～30 ℃;T1、T4最适pH为4～5,G2、G3最适pH为7～8;碳氮比值变化对T1、T4影响不大,而G2、G3的生长受到高碳氮比值的抑制。
Different iso1ated mediums were used to iso1ate cellulo1ytic microbes from different soil,and four fungi strains (Trichoderma T1 and T4;Chaetomium G2 and G3) were got. The analysis of bio-chara- cteristics of which showed that the optimum natural media of Tl, T4 and G2 were carrot juice and tomato juice,G3 was spinage juice and PDA;the optimum synthesized media of Tl and T4 were Mayer and Boas respectively, G2 and G3 were Mayer and Hopkin;the optimum carbon resources of Tl and T4 were fructose and maltose respectively,G2 and G3 are the same as maltose;the optimum nitrogen recourse of Tl and T4 was peptone,G2 and G3 were benefited form the cake powder;the optimum growth condition was under 25～30 ℃,pH 4～5;but il1umination had little effect on the growth of these four strains;the changed ratio of carbon to nitrogen had little affection on Tl and T4,but the higher ratio of carbon to nitrogen did restrain the growth ability of G2 and G3.
出处
《黑龙江八一农垦大学学报》
2004年第1期75-78,共4页
journal of heilongjiang bayi agricultural university
