摘要
目的 :分离制备陆英中的乌索酸 ,并建立其含量测定方法 方法 :采用“醇提凝析法”分离制备乌索酸 ,HPLC法测定其含量 色谱柱为SymmetryShieldRP 1 8,流动相为甲醇 -水(88∶1 2 ) ,流速 1ml/min ,检测波长 2 2 0nm ,柱温 2 5℃ 结果 :乌索酸在 4 6～ 2 3 0 μg范围内线性关系良好 (r=0 9999) ,平均回收率为 99 82 % (RSD为 0 75 % ) 结论 :“醇提凝析法”分离制备乌索酸工艺先进 ,实用可行 ;HPLC法测定乌索酸含量准确、可靠 。
Objective:To isolate and prepare ursolic acid from Sumbucus Chinensis Lindl,and to build the method of determining its content Methods:Adopting the method of ethanol extract and agglomerate separate to isolate ursolic acid The content of ursolic acid in sample is determined by HPLC with methanol-H 2O(88∶12) as mobile phase at a flow rate of 1 ml/min, and detection wavelength at 220 nm A SymmetryShield RP18 column is used at 25℃ Results:A good linearity can be obtained in the range of 4 6～23 0μg(r=0 9999)for ursolic acid The average recovery of ursolic acid is 99 82% with RSD of 0 75% Conclusion:The technology of isolating and preparing ursolic acid is advanced,practical and feasible The content determination is accurate and reliable,and can be used to determine the content of ursolic acid
出处
《宜春学院学报》
2003年第6期1-3,共3页
Journal of Yichun University
基金
国家"8 6 3"计划重点资助项目 ( 2 0 0 2AA2Z32 1 7)
关键词
陆英
乌索酸
分离
制备
高效液相色谱法
含量分析
HPLC
Sumbucus Chinensis Lindl
ursolic acid
Separate and prepare
HPLC
content determination
