摘要
目的 探讨不同方法制作兔VX2 移植性肝癌模型的特点 ,评价超声检查在监测VX2 肝癌中的价值。方法 将VX2 细胞滤液、VX2 瘤株小块及VX2 瘤株小块 +明胶海绵分别注射或接种于 2 8只新西兰大白兔的肝脏内 ,一周后不同时间行超声检查。结果 A组滤液注射法 :平均成瘤时间约 2 1天 ,肝的原位成瘤率为 75 %。B组包块埋植法 :平均成瘤时间14天 ,肝的原位成瘤率为 83 %。C组包块埋植 +明胶海绵法 :平均成瘤时间 14天 ,肝的原位成瘤率 88%。三组成瘤率统计学无显著差异 ( χ2 =1.118,P >0 .0 5 )。肝脏内的移植瘤在超声上均表现为低回声伴声晕的肿块 ,彩色多普勒血流成像表现为周边血供丰富 ,中央血供稀少。结论 三种方法均能制成肝癌模型 ,超声检查是一种监测肝癌模型的有效手段。
Objective To discuss three methods of making rabbit model bearing VX 2 liver tumor and to evaluate the application of ultrasound in evaluating tumor model. Methods Twenty-eight New Zealand white rabbits were inoculated with cell suspension or a small piece of or a small piece with gelatin sponge of VX 2 tumor in the left lobe of liver. Series of ultrasound scanning were performed in the experimental animals after 1 week of implantation or injection of VX 2 tumor. Results The growth rate of neoplasia was 75% with a mean time of 21 days in group A and 83% with a mean time of 14 days in group B and 88% with a mean time of 14 days in group C separately. There was no difference in three groups (χ2=1.118, P> 0.05). All the tumors are low echo companied with dark circle which are hypervascular showed by CDFI. Conclusion All three methods can be used in making rabbit model bearing VX 2 liver tumor. US is useful in evaluating liver tumor.
出处
《中国医学影像技术》
CSCD
2003年第12期1603-1605,共3页
Chinese Journal of Medical Imaging Technology
