摘要
目的探讨开腹直视瘤块接种法及超声引导下肿瘤单细胞悬液接种法制作兔VX2原位肝癌模型的特点。方法选择新西兰大白兔16只,随机分为开腹组及超声介入组,每组8只;分别在开腹直视下将瘤粒接种于兔肝内及超声引导下将单细胞悬液注入肝内,1周后间断行超声检查,动态观察肿瘤生长情况及有无并发症等,并对两组动物超声与病理检查结果进行对比分析,评价超声在兔VX2原位肝癌模型制作中的应用价值。结果开腹组与超声介入组原位种植分别为7只与6只,无明显差异。虽然开腹组平均成瘤时间早于超声介入组,但是超声介入组实验操作简单、省时,且并发症明显少于开腹组,分别是1只(超声介入组)和7只(开腹组)。接种后期(>28d)均出现肝内转移和远处转移,分别为8个(超声介入组)和14个(开腹组)转移灶。且可以用超声实时监测肿瘤的大小、形态、回声、血流情况等。结论超声引导接种制作的兔VX2原位肝癌模型是超声介入治疗研究的理想动物模型,超声检查可作为监测兔VX2原位肝癌模型的一种简便准确的影像学方法。
Objective To explore feature of two methods by percutaneous ultrasonic guidance and via laparotomic route in establishing rabbit model bearing VX2 liver tumor and to evaluate the application of ultrasound in monitoring the tumor model. Methods Sixteen New Zealand white rabbits were randomly classified into two groups incuding eight rabbits in each group. A piece of tumor tissue was implanted into the liver by laparotomic route in group 1. VX2 tumor cell suspension was injected into the rabbit liver by percutaneous uhrasounic guidance in group 2. Aseries of ultrasound scanning were performed in the experimental rabbits after 1 week. The sizes of hepatic VX2 tumor and complications were compared between the two groups. Results Tumors were successfully implanted into 7 rabbits in group 1 and 6 rabbits in group 2 respectively. There were no apparent differences between the two groups. Compared with group 2, the complications were scare, the establishment of model was easier and the time consuming was less in the group 1. Conclusion The percutaneous ultrasonic guidance to make rabbit model bearing VX2 liver tumor can be a desirable animal model for the research of ultrasonic intervention. The uhrasound examination is a simple and accurate method for evaluating the VX2 tumor.
出处
《中华医学超声杂志（电子版）》
2009年第1期73-75,共3页
Chinese Journal of Medical Ultrasound(Electronic Edition)
基金
福建省卫生厅青年科研课题资助项目(2007-1-5)
关键词
肝肿瘤
动物模型
兔
超声检查
Liver neoplasns
Aninal models
Rabbits
Uhrasonography
