摘要
用免疫细胞化学与双染技术对 5 HT和GnRH及其受体在长牡蛎卵巢中的分布的研究结果揭示 ,不同发育时期卵巢中卵母细胞均存在 5 HT和GnRH受体 ,受体阳性物质沿胞膜分布 ,显深棕色 ,核显免疫阴性反应 ;5 HT和GnRH及其受体共存于不同时期卵母细胞胞质和胞膜上。以上结果表明长牡蛎的卵母细胞不仅能产生 5 HT和GnRH ,而且能表达 5 HT和GnRH的受体 ,5 HT和GnRH可能以自分泌的方式对卵母细胞起调节作用。
Distribution of 5-HT,GnRH and their receptors within ovaries of Crassostrea gigas was studied using the immunocytochemistry and double staining technique.The results revealed that ①5-HT and GnRH receptors existed in the oocytes of different development stages of the ovary;②the reactive substances of 5-HT and GnRH receptors were distributed along the membrane of oocytes and showed dark brown,but nucleus showed immunonegative reaction;③5-HT,GnRH and their receptors co-existed in the cytoplasm and membrane of oocytes of different development stages.The above results indicate that the oocytes of Crassostrea gigas can not only produce 5-HT and GnRH,but also express their receptors.5-HT and GnRH may exert regulation action on the oocytes by autosecretion.
基金
福建省重中之重资助项目
