摘要
目的 对张家界市食用菌样品中分离的唐菖蒲伯克霍尔德氏菌进行病原特征、毒力基因检测以及米酵菌酸分析,了解该菌在本地区食用菌中污染特征,为食品安全风险监测提供科学依据。方法 参照《2024年国家食品污染物和有害因素风险监测工作手册》对2024年从张家界市农贸市场和超市采集的木耳与蘑菇共50份食用菌进行唐菖蒲伯克霍尔德氏菌的分离鉴定、产毒基因和米酵菌酸检测。结果 50份样品中共11份样品(22.00%)检出唐菖蒲伯克霍尔德氏菌,其中3份样品中该菌检出bon毒力基因阳性,bon毒力基因阳性的样品由湖南省疾病预防控制中心检测米酵菌酸,其中1份样品为阳性。结论 唐菖蒲伯克霍尔德氏菌易在食用菌中生长,且有产米酵菌酸风险,需加强对重点食品中该致病菌的风险监测,尤其是携带bon毒力基因菌株的检测。
Objective To analyze the pathogenic characteristics,virulence genes and bongkrekic acid of Burkholderia gladioli isolated from edible fungi samples in Zhangjiajie City,to understand the contamination characteristics of this bacterium in local edible fungi,and to provide a scientific basis for food safety risk monitoring.Methods With reference to the National Work Manual for Risk Monitoring of Contaminants and Harmful Factors in Food in 2024,isolation and identification of Burkholderia gladioli,and detection of toxin-producing genes and bongkrekic acid were performed on 50 edible fungi samples including Auricularia auricula and mushrooms collected from farmers’markets and supermarkets in Zhangjiajie City in 2024.Results Burkholderia gladioli was detected in 11(22.00%)out of 50 samples,with 3 samples being positive for the bon virulence gene of this bacterium.The samples positive for the bon virulence gene were analyzed for bongkrekic acid by Hunan Provincial Center for Disease Control and Prevention,and 1 of them was positive.Conclusion Burkholderia gladioli tends to grow in edible fungi and has a high risk of producing bongkrekic acid.It is necessary to strengthen risk monitoring of this pathogenic bacterium in key food products,especially the detection of strains carrying the bon virulence gene.
作者
王敏
高琼
WANG Min;GAO Qiong(Zhangjiajie Municipal Center for Disease Control and Prevention,Zhangjiajie,Hunan 427000,China;Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410153,China)
出处
《实用预防医学》
2025年第8期1004-1006,共3页
Practical Preventive Medicine
