摘要
[目的 ]探讨上海地区老年期痴呆与载脂蛋白E (APOE)ε4等位基因之间关联。 [方法 ]采用聚合酶链反应和限制性片段长度多态性分析技术 ,对 70例老年性痴呆 (AD)患者和 5 8例血管性痴呆 (VD)患者及 5 5位健康老人的APOE基因进行分型 ,计算基因频率 ,并验证APOE基因多态性与疾病的关联性。 [结果 ]APOEε4等位基因与AD显著相关 ,OR =2 83 (P <0 0 5 )。AD患者的ε4基因频率较正常对照及VD患者明显增高 ,三组APOE基因频率分别为 2 5 0 %、10 0 %及 11 2 % ,且随着ε4基因剂量增加 ,AD患病率明显增高 ,发病年龄下降。APOEε4等位基因与VD无关联。 [结论 ]本研究结果提示 ,APOEε4等位基因与AD发病有关 ,可能是AD发病的危险因子之一。
To explore the association of senile dementia with the apolipoprotein Eε4 allele in Chinese Han population. 70 cases with Alzheimers disease(AD),58 cases with Vascular Dementia(VD) and 55 normal controls were detected by polymerase chain reaction and DNA restriction fragment length polymorphism,collected to count the frequency of the apolipoprotein E(APOE) alleles,and verified the association of APOE gene polymorphism with the disease. The association analysis suggested that there was a genetic marked association of APOEε4 allele with Alzheimers disease(AD). The frequency of ε4 gene in AD patients was significantly higher than in VD patients and normal controls. Furthermore,the susceptibility to AD increased and the age of onset decreased with the increment of ε4 gene dose. There was no association of APOEε4 allele with VD. [Conclusion] APOEε4 allele is associated with Alzheimers disease. The APOEε4 gene is one of the risk factors of AD.
出处
《环境与职业医学》
CAS
北大核心
2002年第4期213-215,共3页
Journal of Environmental and Occupational Medicine
