摘要
目的 研究参与有机磷农药代谢的羧基酯酶的多态与有机磷接触者易感性的关系。方法 用聚合酶链反应-限制性片段长度多态性法(PCR RFLP)确定75例长期接触有机磷农药工人的丁酰胆碱酯酶(BChE)和对氧磷酶(PonE)的基因型,以症状积分和乙酰胆碱酯酶(AChE)活力(mmol·h-1·ml-1)作为反映其健康状况的指标。分析单基因位点多态工人的健康状况,确定其易感基因型,进行多基因综合分析,考虑年龄、性别以及接触时间的影响,建立多元线性回归方程。结果 BChE基因K位点(BChE K)正常纯合子(6 1例)、杂合子(12例)和异常纯合子(2例)的AChE活力为10 5 .0±2 3.0、84 .4±16 .4和79.0±9.9,症状积分为3.7±3.8、9.2±3.0和12 .5±0 .7;PonE 192位(PON 192 )正常纯合子(37例)、杂合子(2 7例)和异常纯合子(11例)的AChE活力为116 .8±15 .1、91.2±15 .6和72 .3±2 1.4 ,症状积分为2 .0±3.2、6 .7±3.3和9.7±1.8;PonE 5 5位(PON 5 5 )正常纯合子(70例)、杂合子(5例)的AChE活力为10 2 .4±2 3.0、82 .8±2 2 .0 ,症状积分为4 .5±4 .2、9.2±3.6。3个位点的杂合子和(或)异常纯合子与正常纯合子的症状积分差异都有统计学意义(P <0 .0 5 ) ;异常纯合子的症状积分最高、健康状况最差。3个基因位点没有交互作用;
Objective To study the relationship between the polymorphism of butyrylcholinesterase(BChE,EC 3.1.1.8) and paraoxonase(PonE,EC 3.1.8.1),and the individuals' genetic susceptibility to organophosphates pesticides(OPs) exposure.Methods 75 OPs exposure workers were selected to determine their BChE-K , PON -192 and PON -55 genotypes using PCR-RFLP.The accumulative symptom scores and the whole blood acetylcholinesterase activity(mmol·h -1 ·ml -1 ) were used as health index.Firstly,the health condition related to single gene site of the three gene loci was analyzed to determine which kinds of genotype were susceptible.Then,used the multiple variance analysis was to see if there existed interactions among these three gene loci.Finally,established the multi-factor linear regression equation,in which considered some other factors that might influence the health situation such as age,gender and the exposure time.According to the equation,could get the accumulative symptom scores of each kind of subpopulation of different genotypes. Results The mean AChE activities of the exposed workers with BChE-K genotype UU(61 cases),genotype UK(12 cases) and genotype KK(2 cases) were 105.0±23.0,84.4±16.4,79.0±9.9,respectively;The accumulative symptom scores were 3.7±3.8,9.2± 3.0 ,12.5±0.7,respectively.The AChE activities of the exposed workers with PON -192 genotype BB(37 cases),genotype AB(27 cases) and genotype AA(11 cases) were 116.8±15.1,91.2±15.6,72.3±21.4,respectively;The accumulative symptom scores were 2.0±3.2,6.7±3.3,9.7±1.8,respectively.Similarly,the AChE activities of the exposed workers with PON -55 genotype LL(70 cases) and genotype LM(5 cases) were 102.4± 23.0 , 82.8± 22.0;The accumulative symptom scores were 4.5±4.2,9.2±3.6,respectively.Single variance analysis showed that the accumulative symptom scores of the individuals with abnormal homozygote of these three gene loci were the highest,which indicated that they were most susceptible to OPs.Multiple variance analysis showed there were no interactions among the three gene loci.Age,gender and exposure time had no statistical significance,while genotypes of the three gene loci had statistical significance to health situation. Conclusion Genotypes of BChE-K , PON -192 and PON -55 are related to susceptibility to OPs exposure.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2005年第2期83-86,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家"973"重大基础研究项目 (2 0 0 2CB5 12 90 5 )
上海市曙光计划学者资助项目
关键词
羧基酯酶多态
有机磷农药接触者
易感性
疾病遗传易感性
Insecticides,organophosphate
Carboxylic ester hydrolases
Polymorphism,single nucleotide
Genetic predisposition to disease
