摘要
建立基于高效液相色谱(high performance liquid chromatography,HPLC)法测定发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量的方法。用水作为提取剂,乙腈-水混合溶液作为流动相,采用糖柱进行分离,示差检测器检测。当果糖、葡萄糖、蔗糖、麦芽糖及乳糖的添加量在0.2%~10.0%时,加标回收率为92.6%~102.0%,相对标准偏差(n=6)小于3.00%,定量检出限为0.2%。该方法具有快速、准确、稳定性好、灵敏度高等优点,可用于发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量的检测。
A method for the determination of fructose, glucose, sucrose, maltose and lactose in fermented milk drink by high performance liquid chromatography （HPLC） with refractive index detector was developed. The samples were extracted with water. The method was performed on a carbonhydrate column using acetonitrile-water as mobile phase. The average spiked recoveries of fructose, glucose, sucrose, maltose and lactose spiked in the concentration range of 0.2%-10.0% were between 92.6% and 102.0% with relative standard deviation （RSD, n = 6） less than 3.00%. The limit of quantitation was 0.2%. The results indicated that this method is accurate, simple, reproducible and suitable for the analysis of fructose, glucose, sucrose, maltose and lactose in fermented milk drink.
出处
《乳业科学与技术》
2017年第2期23-26,共4页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
关键词
高效液相色谱法
发酵乳饮料
果糖
葡萄糖
蔗糖
麦芽糖
乳糖
high performance liquid chromatography （HPLC）
fermented milk drink
fructose
glucose
sucrose
maltose
lactose
