摘要
采用液相色谱法分析测定玉米粉乳酸发酵饮料中的糖含量 ,以 Hypersil-NH2 为分析柱 ,乙腈 -水为流动相 ,利用示差折光检测器 ,可以将饮料中果糖、葡萄糖、乳糖和多种低聚麦芽糖较好地分离 ,并测定其各自的含量 ,所得结果良好 ,变异系数为 0 .81%～ 2 .5 % ,线性相关系数为 0 .9996～ 0 .9999,回收率为 97.0 %～ 98.5 %。
The method of liquid chromatography is applied to determine the sugar type and content in corn beverage fermented by lactic acid bacteria. With the column of Hypersil NH 2, the mobile phase of acetonitrile water and a differential index detector, various sugar in corn beverage, such as fructose, glucose, lactose and several maltooligoscchrides, were effectively separated, and the corresponding contents were also determined respectively. The coefficients of variation of the determination were 0.81%~2.5%, the linearly dependent coefficients were 0.999 6~0.999 9, and the recovery rates were 97.0%~98.5%.
出处
《合肥工业大学学报（自然科学版）》
CAS
CSCD
2002年第1期147-149,共3页
Journal of Hefei University of Technology：Natural Science
关键词
液相色谱法
玉米乳酸包料
糖含量
测定
liquid chromatography
corn lactic acid beverage
sugar content
