摘要
为测定饮料中异麦芽低聚糖的含量 ,建立了使用 30 0mm× 4 6mm ,5 μmHypersil NH2 柱 ,乙腈 +水 =78+ 2 2为流动相 ,内部温度为 4 0℃的示差检测器的高效液相色谱法。异麦芽糖和异麦芽三糖的回收率范围分别为 88 5 %～ 10 1 0 %、90 1%～ 10 2 0 % ,相对标准偏差 <5 %。异麦芽糖、异麦芽三糖、潘糖的最小检出量为 2、10、5 μg。该方法的试样前处理简单 ,检测灵敏度高 ,分析速度快、准确 。
For determination the content of isomaltooligosaccharide in beverage, a high performance liquid chromatography method was established by using 300 mm×4 6 mm, 5 μm Hypersil NH 2 column, acetonitrile+water=78+22 as mobile phase, detector was diffeential refractometer with 40℃ internal temprature. The range of recoveries of isomaltose and isomaltotriose were 88 5%～101 1%and 90 1%～102 0% respectively, the RSD <5%. The limits of determination were as follow, isomaltose 2 μg, isomaltotriose 10 μg, panose 5 μg. The method had such charactors, simple sanple trestment, high sensetive, rapid and accurate, it could be used in determination of isomatooligosaccharide in beverage. Author's address: Chen Guiru, Hebei Center for Disease Control and Prevention, Baoding Hebei province 071000, PRC.
出处
《中国食品卫生杂志》
2002年第5期14-16,共3页
Chinese Journal of Food Hygiene
基金
卫生部基金资助课题 (课题号 :BJ-0 1-2 0 )
