摘要
研究载脂蛋白E(ApoE)基因多态性与持续性植物状态 (PVS)之间的关系 ,探讨PVS发生的遗传背景及其对血脂水平的影响。以 6 2名PVS患者为研究对象 ,5 5名正常人为对照 ,采用聚合酶链反应和限制性片段长度多态性(PCR RFLP)方法 ,分析了载脂蛋白E基因多态性 :血脂水平按常规酶法进行测定并进行统计学处理。ApoE基因多态分析表明 ,在PVS患者和正常人中观察到 5种ApoE基因型 ,分别为E3/ 3、E3/ 4、E2 / 2、E2 / 3及E4 / 2。PVS患者组ApoE3/ 4基因型频率高于对照组 (χ2 =14 .2 36 ,P <0 .0 0 1) ;而E3/ 3基因型频率较对照组显著降低 (χ2 =5 .348,P <0 .0 5 )。PVS患者的ε4等位基因频率显著高于对照组 (χ2 =10 .5 33,P <0 .0 0 1) ;而ε3等位基因频率显著低于对照组 (χ2 =7.0 2 2 ,P <0 .0 1)。两组ApoE基因型E2 / 2 ,E2 / 4 ,E2 / 3,E3/ 4的低密度脂蛋白胆固醇 (LDL C)水平之间存在统计学差异 (P <0 .0 5 ,P <0 .0 5 ,P <0 .0 5 ,P <0 .0 1)。ApoE基因多态性与PVS有关联 ,并影响患者的血脂水平。
We studied the relationship between apolipoprotein E gene polymorphism and persistent vegetative state (PVS) to explore the genetics background of PVS, and evaluated the effect of ApoE gene polymorphism on lipid levels in plasma. The ApoE genotype of fifty six patients with PVS and fifty three controls were determined by PCR and restriction fragment length polymorphism (PCR RFLP). Plasma lipid levels were measured by using routine methods. Results demonstrated that there were five genotypes in the two groups: E3/3,E3/4,E2/2,E2/3 and E2/4 . The genotype frequencies of ApoE gene in PVS were 21(37.5%),26(46.4%),2(3.6%),5(8.9%),2(3.6%) and that in control were 37(69.8%),7(13.2%),2(3.8%)5(9.4%),2(3.8%) respectively. We compared the genotype frequencies between the two groups and found there was a significantly increase in E3/4 ( χ 2 =14.236, P<0.001) and decrease in E3/3 ( χ 2 =5.348, P<0.05) . ε2 , ε3 and ε4 allele frequencies of ApoE were 11(9.8%),73(65.2%),28(25%) in PVS and were 11(10.4%), 86(81.1%),9(8.5%) in control respectively. Allele frequencies, significantly increased in ε4 ( χ 2 =10.533, P<0.001) and decreased in ε3 ( χ 2 =7.022, P<0.01) . We also found that E3/4,E2/4 genotype and ε4 allele can largely increase total cholesterol (TC) and lower density lipoprotein cholesterol(LDL C) levels in plasma, and ε2 alleles also can largely increase LDL C levels inplasma. Our finding indicates that the ApoE gene polymorphism may be in association with the PVS, and may be a factor in the genetic susceptibility to PVS in Chinese; Genotype and alleles of ApoE in PVS can affect the lipid levels in plasma.
关键词
载脂蛋白E
基因多态性
持续性植物状态
关系
血脂
persistent vegetative state
apolipoprotein E
gene polymorphism
plasma lipid
