摘要
目的建立甘肃不同产地黄芪和红芪药材中总皂苷含量测定的方法,比较不同产地黄芪和红芪总皂苷含量。方法以黄芪甲苷为对照品,采用香草醛-高氯酸显色法,检测波长为540 nm,测定甘肃5县1区28批黄芪和28批红芪样品中总皂苷的含量。结果黄芪甲苷对照品质量在0.0498~0.1638 mg范围内与吸光度线性关系良好（r=0.9993）,黄芪和红芪的平均加样回收率分别为96.87%和98.96%,相对标准偏差分别为2.00%和2.86%（n=6）。甘肃各地区黄芪样品中总皂苷平均含量分别为宕昌5.10%、武都4.41%、陇西等3地4.21%;红芪样品中总皂苷平均含量分别为宕昌3.41%、武都4.15%、陇西等4地3.06%。结论本研究建立的黄芪和红芪总皂苷含量测定方法简便、准确,重复性好,可用于甘肃地区黄芪和红芪中总皂苷含量测定及其对比研究。甘肃不同产地黄芪和红芪药材中总皂苷含量存在差异,宕昌黄芪总皂苷含量最高,武都红芪总皂苷含量最高,且同一地区黄芪总皂苷含量高于红芪总皂苷。
Objective To establish a method for determining the total saponins content of Astragali and Radix Hedysari in Gansu Province, and compare the total saponins content of Astragali and Radix Hedysari in different areas of Gansu Province. Methods The vanillin-perehloric acid colorimetric method was used to detect the total saponins content of 28 batches of Radix Astragali and 28 batches of Radix Hedysari samples which were got from five regions of Gansu Province, Astragalus saponins was used as the control sample and 540 nm was chosen as the detection wavelength. Results The absorbance and the concentration had a good linear relationship （r = 0.9993 ） , and the absorbance of astragaloside IV was linear in range of 0. 0498 - 0. 1638 mg. The average recoveries of Astragali and Radix Hedysari were 96. 87% and 98.96% respectively, the RSD were 2.00% and 2.86% （n = 6） respectively. The average content of the total saponins in the sample of Astragalus in Tangchang was 5.10% , Wudu was 4.41% , Longxi and another two areas were 4. 21%. The average content of total saponins of Radix Hedysari samples in Tangchang was 3.41% , Wudu was 4. 15% , Longxi and another two areas were 3.06%. Conclusion The method for detecting the total saponins content of Astragali and Radix Hedysari in Gansu Province was simple, accurate and reproducible, and can be used for the comparative study on the content of total saponins of Astragalus and Radix Hedysari. There are differences in the content of total saponins of Radix Astragali and Radix Hedysari in Gansu Province. The total saponin content of Astragalus in Wudu is highest, and the total saponin content of Radix Hedysari in Tangchang was the highest. Total saponin content of Astragalus was higher than that of Radix Hedysar in the same area of Gansu Province.
出处
《环球中医药》
CAS
2016年第10期1197-1203,共7页
Global Traditional Chinese Medicine
基金
基金项目:中国中医科学院自主选题(YZ-1438)
关键词
黄芪
红芪
总皂苷
紫外-可见分光光度法
含量测定
Astragalus
Radix Hedysari
Total saponins
Ultraviolet visible spectrophotometry
Content determination
