摘要
目的:建立楮实子总皂苷的含量测定方法。方法:以薯蓣皂苷为对照品,采用分光光度法,高氯酸显色,在407nm处测定吸光度,计算楮实子中总皂苷的含量。结果:薯蓣皂苷的质量浓度在8.04～40.20μg.mL-1与吸光度呈良好的线性关系(r=0.999 8),平均回收率为99.8%(n=9),RSD=2.01%;楮实子中总皂苷平均含量为0.48%(n=10),RSD=1.88%。结论:建立了楮实子中总皂苷含量测定方法,此方法简便、准确,稳定性、重现性好,可用于楮实子的质量控制。
Objective： To establish the determineation methods of total saponins from fructus broussonetia. Methods： Selecting dioscin as control article, colorating with perchloric acid, determining absorbability in the point of 407nm by spectrophotometric method, then calculating total saponins in fructus broussonetia. Results： The calibration curve was linear over the range of 8.04 -40. 20μg· mL^-1 with the correlation of 0. 999 8. The average recoveries of dioscin was 99.8% （ n = 9 ）, RSD = 2. 01%. The contents of total saponins were 0. 48% （ n = 10） ,RSD = 1.88%. Conclusion： The methods were fast,accurate,reliable and suitable for analysis of total saponins. It can be applied to the quality control of fructus broussonetia.
出处
《中国现代中药》
CAS
2009年第7期26-27,44,共3页
Modern Chinese Medicine
关键词
楮实子
总皂苷
薯蓣皂苷
分光光度法
Fructus broussonetia
Total saponins
Dioscin
Spectrophotometric method
