摘要
通过对酵母胞壁不溶性β-D-葡聚糖的几种定量测定方法的比较分析,表明经酸水解法处理后得到的测定结果偏高,经酸-酶水解法处理后的测定结果更准确;对水解后产生的葡萄糖采用生物传感仪法定量,结果稳定,操作时间短,更适合在生产过程和质量控制中使用;待测样品中的α-葡聚糖对测定结果有明显的干扰,β-D-葡聚糖含量应为总葡聚糖含量与α-葡聚糖含量之差。
This study was conducted to compare and analyze several quantitative assay methods for insoluble β-D-glucan from yeast cell walls. It was indicated that the sample pretreatment by acid hydrolysis provided higher results for the determination of β-D-glucan, while the results obtained by acid-enzymatic hydrolysis were more precise. Among different test methods for glucose obtained after hydrolysis, the biosensor method was found to be faster, more stable and suitable for quality control in the production process. Besides, α-glucan in the samples had a significant interference effect on the results of determination. Thus β-Dglucan should be calculated by subtraction from total glucose of the glucose obtained from α-glucan.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第2期99-103,共5页
Food Science
基金
国家高技术研究发展计划(863计划)项目(2012AA021403)
