摘要
采用高效液相色谱法（ＨＰＬＣ），以μ－ＢｏｎｄａｐａｋＣ１８柱为分析柱，水为流动相，示差折光仪为检测器，一次进样能同时测定低聚果糖样品中的果糖、蔗糖、三糖、四糖、五糖、六糖等。测定了“博爱生命素”样品，所得结果良好，变异系数为０．５５％～１．１％，线性相关系数为０．９９９２～１．０００。低聚果糖的最低检出限在微克级。
In this paper, an analytical method for determination of fructooligosaccharide by high performance liquid chromatography(HPLC) is presented. The HPLC conditions are μ Bondapak C 18 column, 300 mm× 3.9 mm i.d., water as mobile phase and refractive index detector. Monosaccharide, disaccharide, trisaccharide,tetrasaccharide, pentasaccharide, hexasaccharide etc. can be simultaneously determined in a single injection. The method has been used to determine the fructooligosaccharides in “Boai biogen”. The results are satisfactory. The CV s were 0.55% 1.1% and correlation coefficients were 0.999 2 1.000. The lowest detectable limit for fructooligosaccharide was in microgram level.
出处
《色谱》
CAS
CSCD
北大核心
1999年第1期87-89,共3页
Chinese Journal of Chromatography
关键词
低聚果糖
组分
测定
HPLC
high performance liquid chromatography, fructooligosaccharide, analysis
