摘要
建立了双黄连粉针剂中黄芩苷含量测定的高效毛细管电泳电导法 ;以未涂层融硅毛细管 (75μm×50cm)为分离柱 ,Tris-H3BO3 为电泳介质 (15mmol/LTris ,1.5mmol/LH3BO3) ,在18kV、pH8.5的碱性环境下 ,柱端电导检测双黄连粉针剂中的黄芩苷含量 ;重点探讨了缓冲溶液的种类、浓度、分离电压、进样时间对检测的影响 ;该法的线性范围为10～720mg/L,检出限为2.5mg/L;结果表明该法简便、快速、结果准确 ,适用于含黄芩苷的制剂含量的测定。
A method for the determination of baicalin in Shuanghuanglian powder _ injectable was established by high performance capillary electrophoresis with conductivity detection. Based on the mode of capillary zone electrophoresis, baicalin was successfully separated from other components and determined within 5 minutes under following selected conditions: uncoated fused silica capillary(75 μm×50 cm), Tris-H3BO3 buffer solution(pH=8.5, 15 mmol/L Tris, 1.5 mmol/L H3BO3), 18 kV operation voltage. The effect of kinds of buffer solutions, buffer solution concentration, operation voltage, sampling time were discussed in detail. A linear range of 10～720 mg/L and a detection limit of 2.5 mg/L for baicalin were obtained. The method proved to be convenient, rapid and well repeatable, and can be used for the quality control of preparations with baicalin.
出处
《分析测试学报》
CAS
CSCD
北大核心
2001年第6期21-24,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目 (29675033)
广东省自然科学基金资助项目 (001237)
